IsCT on grass carp !

Column:Aquatic animals Time:2021-04-29
https://doi.org/10.1016/j.aquaculture.2021.736585

Antimicrobial peptide Isalo scorpion cytotoxic peptide (IsCT) enhanced growth performance and improved intestinal immune function associated with janus kinases (JAKs)/signal transducers and activators of transcription (STATs) signalling pathways in on-growing grass carp (Ctenopharyngodon idella)


ABSTRACT

This dissertation was primarily explored the impacts of antimicrobial peptide (AMP), Isalo scorpion cytotoxic peptide (IsCT), on growth and intestinal immune function of fish. For this objective, 540 fish (136.88 ± 0.72 g) were randomly selected for 6 treatments for a growth trial and fed 6 levels of AMP (0, 0.6, 1.2, 1.8, 2.4, 3.0 mg/kg diet) within 60 days. After that, grass carp were challenged by A. hydrophila for 6 days. This research revealed that appropriate AMP supplementation: (1) increased fish growth performance and enhanced the enteritis resistance. Notably, quadratic regression analysis of PWG and enteritis morbidity, the optimal dose of AMP (IsCT) for on-growing grass carp (136.88–507.78 g) were calculated to be 1.52 and 2.00 mg/kg diet, respectively. (2) improved the intestinal immunity of fish partially resulted from increased above immune substances activities and expression [e.g., acid phosphatase (ACP) β-defensin-1]; (3) attenuated the fish intestinal inflammatory response by up-regulating the studied anti-inflammatory cytokines gene expression and down-regulate the main pro-inflammatory cytokines, which might be partly regulated to janus kinases (JAKs) / signal transducers and activators of transcription (STATs) signalling pathways molecules. However, AMP supplementation did not alter STAT2 and STAT3a gene expression in fish intestines. In conclusion, this study provided evidences that AMP enhanced growth performance and improved intestinal immune function and systematically researched its related pathway (JAKs/STATs signalling pathway).


Antimicrobial peptide Isalo scorpion cytotoxic peptide (IsCT) enhanced growth performance and improved intestinal immune function associated with janus kinases (JAKs)/signal transducers and activators of transcription (STATs) signalling pathways in on-growing grass carp (Ctenopharyngodon idella) - ScienceDirect.

 

For more information please contact us.